Coding
RFC 37

Part:BBa_K245117

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-10-14)

GyrB-SG

The sequence encodes GyrB, a 24 kDa fragment of gyrase subunit B. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. This part can be used to form fusions with extended linker according to assembly standard 37.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None