Coding
RFC 37
Part:BBa_K245117
Designed by: Nika Debeljak Group: iGEM09_Slovenia (2009-10-14)
GyrB-SG
The sequence encodes GyrB, a 24 kDa fragment of gyrase subunit B. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. This part can be used to form fusions with extended linker according to assembly standard 37.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 428
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 100
Illegal AgeI site found at 238 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |